2×Taq Plus PCR Mix

Taq Plus DNA Polymerase is a mixture of Taq and Pfu DNA polymerase. It has 5’-3’ exonuclease activity and 3’-5’ exonuclease activity, with the characteristics of high amplification efficiency and low mismatch rate. Compared with Taq DNA polymerase, Taq Plus DNA polymerase has the advantages of increased amplification length (simple templates can be effectively amplified up to 20kb and complex templates can be up to 10 kb), good fidelity, etc. Compared with Pfu DNA polymerase, it has the advantages of fast amplification speed and high reaction efficiency.
Cat. No Packing Size
4992791 1ml
4992792 5*1ml
4992793 5×1 ml
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Products Details

Activity Definition

1 Unit (U) Taq Plus DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

Taq Plus DNA Polymerase has 5'-3' exonuclease activity and 3'-5' exonuclease activity. PCR products can be directly cloned into TA vector. If the cloning efficiency needs to be improved, it is recommended to purify the PCR products first and perform A-tailing before cloning into TA vector. One-tube Taq Plus PCR Mix (National High-Tech Product Certification) ■ The Taq Plus PCR Mix has improved specificity and sensitivity of PCR reaction and can amplify complex templates with high GC content, secondary structure and the like. As low as 2 copies of the target template can be amplified, ensuring more accurate experimental results. ■ The unique Taq Plus PCR Mix formula makes the whole reaction system very stable, and the activity will not be affected by repeated freeze-thaw or long-term storage at 4°C. ■ The stable and efficient pre-prepared PCR mix solution can make the operation fast and simple, greatly reducing labor intensity and sampling error. High-performance PCR enhancer and optimizer are also included in the mix, which reduces the requirements on PCR conditions. ■ This product has both dye-containing and dye-free systems. Dye-containing PCR Mix products can be directly electrophoresed after PCR, without adding sample buffer.

Applications

It is commonly used for amplification of templates with high fidelity and complex structure, such as high GC content and secondary structure. In most cases, it can replace Taq DNA polymerase.

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