The GMO Crop Extraction & Amplification Kit is specifically developed for PCR detection of GMO crops. The unique lysis buffer contained in Part A of the kit can specifically lyse the tissues of the main crops——wheat, corn, rice, cotton and soybean, to release related components such as nucleic acids and proteins. The phenol/chloroform extraction combined with the specific RNase can purify high-purity genomic DNA with no impurities such as RNA, protein and metal ions. The purified DNA can be applied in subsequent PCR detection. Part B of the kit is a two-component simple PCR reaction system containing 2×GMO PCR Buffer and GMO DNA Polymerase. GMO DNA Polymerase is a thermostable polymerase modified with antibodies. The 2×GMO PCR Buffer contains various components such as MgCl
2, dNTPs, PCR reaction stabilizer, optimizer and enhancer at a concentration of 2×GMO. It has the advantages of fast and simple operation, high sensitivity, strong specificity, good stability, etc. It can be used in combination with Part A for GMO crop transgenic PCR detection.
Cat. No | Packing Size |
4992905 | 200 rxn |