The Mouse Tissue Direct PCR Kit adopts a unique packaging design that includes all the reagents for rapid genomic DNA preparation and PCR amplification. It is applicable for the one-step genome DNA purification from mouse tissues (tail, ear and toe) and the subsequent PCR amplification and detection. The whole process does not include homogenization, smashing, overnight digestion, organic solvent extraction, ethanol precipitation or column purification steps. Stable results can be obtained with simple and fast operations.
The 2× Dir PCR MasterMix provided by this kit is a highly compatible PCR reagent that can efficiently and specifically amplify DNA without the need for removing impurities such as proteins. This reagent contains an antibody modified hot-start Taq DNA polymerase, dNTPs, MgCl2, buffers, as well as the enhancer, optimizer and stabilizer for PCR reaction. The PCR reaction can be performed by simply adding in roughly extracted template and specific primers. The whole process is fast, simple, sensitive, specific and stable, which is especially suitable for high-throughput screening. The 2× Dir PCR MasterMix contains premix electrophoresis dye, so that the PCR products can be directly sent for electrophoresis detection after the reaction. The 3’ end of the PCR product has a A-tailing, which can be used for TA cloning.
| Cat. No | Packing Size |
| 4992531 | 20 µl×50 rxn |
| 4992532 | 20 µl×200 rxn |