QuantScript RT Kit

Quantscript RT Kit (cDNA first strand synthesis kit) is specially prepared for the first step of two-step RT-PCR experiment. The product comprises a brand-new high-efficiency reverse transcriptase Quant Reverse Transcriptase, a reaction buffer and necessary reaction components for RT reaction. Total RNA or mRNA can be used as a template for efficient reverse transcription into complementary cDNA first strand with this kit, and further downstream experiments can be carried out.
Cat. No Packing Size
4992783 20 µl×25 rxn
4992784 20 µl×100 rxn
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Products Details

Features

■ The reverse transcription efficiency can reach 90%. ■ The whole experiment can be completed in one step at 37℃ . ■ RNA template with high GC content and complex secondary structure can be read through. ■ It has good compatibility with subsequent PCR or qPCR experiments and is compatible with various PCR thermostable polymerases.

Applications

■ Suitable for reverse transcription of total RNA template with amount of 50 ng-2 μg. ■ Real-time RT-PCR. ■ Semi-quantitative PCR reaction. ■ 3'- and 5'- RACE, etc.

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