Features
■ High purity: Silica membrane gets rid of most impurities. ■ Wide use: Suitable for tissue, cells, blood and many other kinds of samples. ■ Fast operation: Genomic DNA could be obtained within 1 hour.Specification
Type: Spin column based Sample and starting volume: 100-400 μl whole blood from mammalian, 5-20 μl whole blood from bird or amphibian, 106-107 cultured cells, 30 mg tissue Yield: 3-40 μg Operation time: ~1 hour Downstream applications: Restriction enzyme digestion, PCR analysis, Southern blotting, DNA library, etc.Important Notes
■ Please use fresh samples to ensure the integrity of the purified gDNA. ■ If the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of Buffer GA and Buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns. ■ For long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng/μl. DNA Yield from Different Samples and Amounts: