TIANScriptⅡ RT Kit

The kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.
Cat. No Packing Size
4992910 20 µl×25 rxn
4992911 20 µl×100 rxn

Products Details

Features

■ High enzyme activity and efficiency: High reverse transcriptase activity and good compatibility in subsequent experiments. ■ Wide substrate range: Suitable for all RNA, especially RNA templates with complex secondary structures. ■ Long RT length: The synthesis of the cDNA first strand can reach 12 kb. ■ Simple operation: Simply add the required components in one step without adding any reagent during the operation.

Applications

■ Synthesis of cDNA first strand. ■ Construction of cDNA library. ■ One-step RT-PCR. ■ RACE analysis.

Contact us

Please feel free to give your inquiry in the form below We will reply you in 24 hours