The kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.
Cat. No | Packing Size |
4992910 | 20 µl×25 rxn |
4992911 | 20 µl×100 rxn |